Biological Effects of Histamine Dilutions Beyond Avogadro’s Number

Biological Effects of Histamine Dilutions Beyond Avogadro’s Number
New cytometry findings from J. Saintelaudy and co-authors described

“According to a study from Limoges, France, ‘At the beginning of this series of experiments we were looking for a model based on the use of purified commercially available compounds based on a fully described and accepted pharmacological model to study of the biological effect of high dilutions. Negative feedback induced by histamine, a major pro-inflammatory mediator, on basophills and mast cells activation via an H2 receptor me these criteria.’

“‘The simplest way of measuring basophil activation in the early 1980's was the human basophil activation test (HBDT). Our major goal was first to study the biological effect of centesimal histamine dilutions beyond the Avogadro limit, on the staining properties of human basophils activated by an allergen extract initially house dust mite, then an anti-IgE and N-formyl-Met-Leu-Phe (fMLP). Technical development over the 25 years of our work led us to replace the manual basophil counting by flow cytometry....”